Comparison of NFL levels in plasma and CSF measured at two different NFL laboratory facilities
| Site 1 | Site 2 | P value | Site 1 | Site 2 | P value | |
| CSF BL n=30 | CSF BL n=25 | CSF 12 n=35 | CSF 12 n=29 | |||
| Mean | 2368 | 2879 | >0.99 | 644 | 604 | >0.99 |
| SD | 1947 | 1989 | 576 | 472 | ||
| SE | 356 | 398 | 97 | 88 | ||
| P BL n=47 | P BL n=44 | P 12 n=47 | P 12 n=42 | |||
| Mean | 16 | 20 | >0.99 | 7.4 | 8.3 | >0.99 |
| SD | 14 | 16 | 3.1 | 4.5 | ||
| SE | 2.1 | 2.3 | 0.5 | 0.7 | ||
| P HC n=22 | P HC n=23 | CSF HC n=23 | CSF HC n=23 | |||
| Mean | 7.3 | 5.1 | >0.99 | 417 | 283 | >0.99 |
| SD | 3.0 | 2.1 | 191 | 117 | ||
| SEM | 0.6 | 0.4 | 40 | 24 |
Dunn’s multiple comparisons test comparing the levels of NFL (pg/mL) in CSF (total number 165) and plasma (total number 225), between two sites. Samples were from patients with multiple sclerosis, before and after 12 months of treatment with dimethyl fumarate and a HC group. Site 1 and site 2 are two independent NFL laboratories. P12 and CSF12=samples at 12 months.
BL, baseline samples; CSF, cerebrospinal fluid; HC, healthy control; n, number; NFL, neurofilament light chain; P, plasma.