Autonomic Function Tests: Some Clinical Applications

Tests of sudomotor function

The tests are summarized in Table 2, the system evaluated, neural pathways involved, and clinical interpretation. We provide here a brief description of each test.

Table 2
Tests of autonomic function

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Tests of cardiovagal function

Cardiovagal function can be evaluated by a number of methods. In the time domain, the commonly used and most reliable approach is to quantify heart rate response to deep breathing and to the Valsalva maneuver. The subject breathes at 6 breaths per minute and the magnitude of heart rate variation (maximal heart rate minus minimum heart rate) is averaged and provides an index of cardiovagal function. There are a number of alternative approaches, including mean circular resultant.6

The Valsalva ratio is derived from the maximum heart rate generated by the Valsalva maneuver divided by the lowest heart rate occurring within 30 seconds of the peak heart rate.2, 4, 7 Our studies suggest that 40 mm Hg for a duration of 15 seconds should be used as the standard since it has yielded the most reproducible results.8

Cardiovagal function can also be quantified in the frequency domain. Spectral analysis of resting heart rate produces several peaks. The highest frequency peak (>0.15 Hz) reflects oscillations of heart rate due to respiratory sinus arrhythmia and is considered to be a measure of cardiovagal function.6

Tests of adrenergic function

The evaluation of adrenergic function, specifically the vagal and adrenergic components of the baroreflex, can be made by studying dynamic alterations during the Valsalva maneuver.9 There are four main phases in the Valsalva maneuver (Fig. 1). Phase I is a transient rise in BP due to the act of blowing. Early phase II is due to reduced preload (venous return). The baroreflex then results in efferent sympathetic discharge to muscle resulting in a rise in BP. This sustained rise in BP is interrupted by a transient fall in BP, phase III, which like phase I, is mechanical, lasting 1-2 seconds, during which BP falls (because the subject stops the maneuver). This rise in BP is described as phase II_L, BP recovery following phase III and phase IV, reflects the increase in total peripheral resistance (Fig. 1A). Evaluation of the baroreflex can be done non-invasively from the Valsalva maneuver. The vagal component of the baroreflex is evaluated by relating the beat-to-beat BP during phase II_E to the heart period (reciprocal of heart rate) providing baroreflex sensitivity in msec/mm Hg.10 The adrenergic component of the baroreflex can be evaluated by BP recovery time (PRT), which is the time (in seconds) that the BP takes to recover from phase III to baseline.11 Fig. 1B is a Valsalva maneuver from a patient with diabetic autonomic neuropathy. Note that, in contrast with a normal response (IA), heart rate response to the fall in BP (phase II_E) is blunted (indicating impaired vagal baroreflex function). Adrenergic baroreflex function is markedly impaired. This is manifested by the absence of either II_L and IV (absence of reflex vasoconstriction) and prolonged PRT.

Fig. 1
Valsalva maneuver from a normal subject (A) and a patient with diabetic autonomic neuropathy (B). The heart rate and beat-to-beat blood pressure responses to the Valsalva maneuver are shown. Expiratory pressure is shown at the bottom. The phases of the Valsalva maneuver (I, II_E, II_L, III, IV) are indicated in the Fig. 1A. Autonomic neuropathy with adrenergic failure is manifested as a loss of phases II_L and IV and delayed blood pressure recovery. Impairment of the vagal component of the baroreflex is manifested as blunting of heart rate response to changes in blood pressure.

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HUT complements the beat-to-beat BP responses to the Valsalva maneuver. HUT provides an evaluation of the BP and heart rate response to tilt. A normal response consists of a modest rise in heart rate (by 5-20 bpm) and BP remains relatively constant (modest fall in systolic BP by <10 mm Hg and modest diastolic rise). HUT is done to detect if OH is present. Fig. 2 are examples of HUT showing a normal response (Fig. 2A), neurogenic OH (Fig. 2B), POTS in Fig. 2C, and syncope (Fig. 2D). In Fig. 2B, there is a marked fall in BP with a blunted heart rate response, typical of neurogenic OH. This was recorded in a patient with MSA. Fig. 2C is a recording from a patient with POTS, showing orthostatic tachycardia without OH. Heart rate increment is >30 bpm and eventually exceeds 120 bpm. Fig. 2D shows a patient with vasodepressor syncope. To HUT (first mark) there was a transient increase in BP (sympathetic surge) followed by abrupt fall in BP.

Fig. 2
Head-up tilt (HUT) responses in a normal subject (A), neurogenic OH (B), POTS (C), and syncope (D). Neurogenic OH is manifested as a pronounced fall in blood pressure (BP) with a blunted heart rate (HR) response. POTS (C) is manifested as an exaggerated HR response without OH. D shows vasodepressor syncope manifested as an abrupt fall in BP. OH: orthostatic hypotension, POTS: postural tachycardia syndrome.

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Plasma norepinephrine measured with the subject supine and after a period of standing provides another method of studying adrenergic function.12 A normal response consists of doubling of NE on standing. The patient with generalized postganglionic adrenergic failure, as in pure autonomic failure (PAF), will have low supine NE. The patient with preganglionic lesion, as in MSA, will typically have normal supine values (since the postganglionic fibers are intact) but a failure to increment on standing.13

The quantitative uptake of the radiopharmaceutical [¹²³I]MIBG (iodine-123 meta-iodobenzylguanidine), a norepinephrine analog, can be measured by single photon emission computed tomography. A newer approach, using 6-[¹⁸F]fluorodopamine positron-emission tomography and neurochemical analyses provide better resolution. These approaches provide an index of the functional integrity of presynaptic postganglionic adrenergic sympathetic terminals in the heart. Cardiac adrenergic imaging is reduced in a postganglionic lesion as in PAF or PD and is usually normal in MSA, although not invariably.14

Distal small fiber neuropathy

One of the most common and distressing neurologic complaints is DSFN. The patient complains of a burning sensation in their feet, especially toes and plantar aspect of their feet. There is typically troublesome allodynia, where a non painful stimulus elicits pain. Sensory examination may demonstrate a loss of sharp/dull or temperature perception at the toes. Motor function is usually normal. Common causes are diabetic and inherited neuropathy, but the most common cause is idiopathic. Nerve conduction studies are often normal since this is a small fiber and not large fiber neuropathy. This condition causes a length-dependent involvement of unmyelinated fibers, both somatic and autonomic. Sympathetic sudomotor fibers are affected, so that QSART will show abnormalities at the feet and normal sweating more proximally (Table 3) in about 3 out of 4 patients tested. Similarly, the thermoregulatory sweat test shows anhidrosis that is confined to the distal feet. Skin biopsy and evaluation of intraepidermal fiber density on the same subjects and the same sites have been studied prospectively in patients with autonomic neuropathy.15 Both skin biopsy and QSART will accurately diagnose DSFN but their agreement is imperfect, since they measure different populations of unmyelinated fibers.15 QSART is also helpful in following the natural history of DSFN and to determine if the neuropathy remains confined or becomes generalized. This capability is of importance since neuropathies due to diabetes or amyloidosis can start with DSFN and progress, while others do not. It is often difficult to make this determination with clinical examination alone. Table 3 provides a summary of experience with autonomic function tests in DSFN. Either TST of QSART will detect distal denervation in >70% of cases and together show an abnormality in 9/10 cases. As expected, adrenergic and cardiovagal abnormalities are less common. EMG is normal in >70% of cases.

Table 3
Comparison of current study with earlier studies33

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Generalized autonomic failure

Physicians have learnt to recognize symptoms of autonomic failure. These include orthostatic lightheadedness, syncope, erectile dysfunction, and symptoms suggestive of neurogenic bladder and bowel. However, it is not possible to quantitate the severity and distribution of such abnormalities to help the physician determine if the patient is getting better or getting worse or if they are responding to therapy. With the availability of autonomic testing, it is possible to determine if generalized autonomic failure is present. Generalized autonomic failure refers to autonomic failure that is diffuse (multiple regions) and involves more than one autonomic system. Examples of generalized autonomic failure are the autonomic neuropathies16 and multiple system atrophy. Examples of autonomic neuropathies are diabetic autonomic neuropathy, amyloid neuropathy,17 and autoimmune autonomic ganglionopathy.18 In severe diabetic autonomic neuropathy,19 there is widespread loss of sweating, cardiovagal failure is present, and OH with impaired baroreflexes is seen. In the synucleinopathies (Parkinson's disease, multiple system atrophy, Lewy body dementia), generalized autonomic failure tends to be present in MSA, mild in PD, and intermediate in DLB.20

Typically, for a comprehensive evaluation of autonomic function, we undertake an autonomic reflex screen and thermoregulatory sweat test as a routine. We often measure norepinephrine supine and standing to determine if supine values are reduced (indicating widespread postganglionic adrenergic denervation) and if an orthostatic increment (doubling) occurs. We will typically do additional studies to seek a cause. These include fat aspirate or nerve biopsy for amyloidosis17 or an antibody panel seeking especially for presence of ganglionic antibody.21 Detailed descriptions are available for a comprehensive coverage.12

Selective autonomic failure

The astute clinician can suspect that autonomic failure may be restricted rather than generalized. This suspicion can be confirmed and quantified with autonomic testing. Autonomic tests can confirm that a specific autonomic function is affected and that other systems are intact. We provide 3 examples. A patient, aged 30 years, feels hot, weak, dizzy and uncomfortable when ambient temperature rises. BP supine and standing is normal. He looks hot and flushed but has dry skin. Orthostatic BP recording is normal without evidence of OH. The clinician may suspect this is restricted autonomic failure but testing is necessary. Autonomic testing demonstrates normal cardiovagal and adrenergic function. However, QSART and TST show global anhidrosis. This is a condition called chronic idiopathic anhidrosis.1 Another example is a patient with gastroparesis. This patient has postprandial bloating and weight loss. He vomits up food that is 3 days old. Autonomic testing shows normal sudomotor and adrenergic function. Gastric transit studies show marked delay in gastric transit. A third patient has Adies pupils. Tests can be done to confirm the entity, but autonomic testing is needed to demonstrate that the deficits are confined and not generalized.

The synucleinopathies

The term synucleinopathies is used to describe several neurodegenerative disorders characterized by fibrillary aggregates of alpha-synuclein protein in oligodendroglia (in MSA) and in selective population of neurons. These disorders include Parkinson's disease (PD), dementia with Lewy bodies (DLB), PAF, and MSA. Although it is recognized that autonomic failure, manifest as erectile dysfunction, neurogenic bladder, constipation, and OH occur in the synucleinopathies, there has been little emphasis on the use of autonomic function tests in the differential diagnosis of these conditions. Based on our research on the involvement of autonomic structures in brain and spinal cord, we hypothesized that the severity and distribution of autonomic failure was different among synucleinopathies.

PD and MSA share clinical features of hypomimia, hypertonia, dysarthria, micrographia, and gait impairment. PD is more likely associated with rest tremor and is typically levodopa-responsive. However, MSA can also have rest tremor and levodopa responsiveness, albeit less robust.22 Recent studies indicate that MSA is distinguishable from PD using autonomic tests. PD is characterized by a length-dependent involvement of postganglionic sudomotor fibers, whereas MSA is characterized by widespread, early and preganglionic autonomic failure.23 MIBG or fluorodopa scan of the heart, which images postganglionic adrenergic innervation, is typically defective in PD and normal in MSA.13, 14 However, denervation can occur in 25-30% of MSA patients.14 Another useful test is the TST. TST is either normal or shows very distal anhidrosis, typically affecting the toes (Fig. 3). In Fig. 3, the PD case showed very distal anhidrosis, affecting only parts of the toes, and did not progress over time. In contrast, MSA causes widespread anhidrosis.23 If both QSART and TST are performed, normal QSART volume in an anhidrotic region indicates that the lesion is preganglionic in site (Fig. 3). When the autonomic reflex screen is performed and the aggregate score, the composite autonomic severity score (CASS) calculated, CASS separates MSA from PD with sensitivity and specificity. The autonomic and clinical characteristics of the synucleinopathies are distinctive (Table 4). PAF is characterized by severe OH, without clinical central involvement. Supine plasma norepinephrine is often markedly reduced and CASS is >6 and TST% >40%. PD is associated with normal NE, CASS <6, and TST% <40%. MSA is characterized by CASS >6 and TST% >40%. DLB has intermediate autonomic failure.

Fig. 3
Thermoregulatory sweat test (TST) showing characteristic patterns in the synucleinopathies. In Parkinson's disease (top panel), anhidrosis is distal and percent anhidrosis remained <5%. In contrast, in multiple system atrophy (MSA), anhidrosis is regional and percent anhidrosis is greater and progressed more rapidly.

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Table 4
Autonomic scores in the synucleinopathies

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Orthostatic intolerance

Orthostatic intolerance refers to the development of symptoms after assuming the standing posture that clears on sitting or lying back down. Typical symptoms are lightheadedness, blurred vision, cognitive difficulties, or tiredness. Some patients also have symptoms of sympathetic activation, such as tremulousness and palpitations. In the category of orthostatic intolerance, we consider OH,9 postural tachycardia syndrome,24 and neurocardiogenic syncope (vasovagal and vasodepressor).25

Orthostatic hypotension is defined as an orthostatic fall in SBP by >20 mm Hg,26 although in the laboratory, we have routinely required a fall of >30 mm Hg9, 27 and this latter criterion is more robust in the diagnosis of MSA.28 Orthostatic hypotension can occur in the absence of autonomic failure and the role of the laboratory is to determine if it is neurogenic; i.e., if autonomic reflexes, especially the baroreflexes are impaired. Typically in neurogenic OH, the fall in BP is associated with attenuated heart rate increment (Fig. 2B).

POTS is defined as an increase on tilt of heart rate >30 bpm within 5 minutes in adults and associated with symptoms of orthostatic intolerance.24, 29 Autonomic laboratory testing is essential to make the diagnosis of POTS to demonstrate this heart rate increment and to rule out OH (Fig. 2C).

Neurocardiogenic syncope typically occurs in subjects who have normal autonomic reflexes. The most common type is vasovagal syncope. There is a sudden fall in BP and heart rate. There is often an antecedent sympathetic surge as might occur in a subject who gets an injection or sees something unpleasant. Hence patients with POTS, who typically have an increased sympathetic tone, are excessively prone to vasovagal syncope. Vasodepressor syncope refers to an event where there is this fall in BP without an associated fall in heart rate (Fig. 2D). The autonomic laboratory will often capture syncope, although most autonomic laboratories do not attempt to provoke syncope.